Dr Salehghamari en

 

Ensieh Salehghamari

 

Assistant Professor in Microbiology

 

Microbiology Lab.

Dept. of Cell and Molecular Biology

Faculty of Biological Science

University of Kharazmi

Karaj-Iran

E-mail: ensiehsalehghamari@gmail.com

 

 

Educational Background:

 

(2006- 2012)                  PhD in Molecular Microbioloy at Department of Microbiology, School of Biology, College of Science, University of Tehran.

(2003- 2006)                        Master of Science in Environmental Microbiology at Department of Microbiology, School of Biology, College of Science, University of Tehran.

(1999-2003)                         Bachelors of Science in Microbiology at School of Biology, College of Science, University of Tehran.

 

 

Academic Honors:

 

  • The first rank among graduates B.Sc. Microbiology, School of Biology, University of Tehran.
  • The third rank among graduates M.Sc. Microbiology, School of Biology, University of Tehran.
  • Second score in PhD exam of Tehran university
  • The Second rank among graduates PhD, Microbiology, School of Biology, University of Tehran


 

Scientific Interests:

 

  • Natural Products
  • Molecular microbiology
  • Actinomycete physiology and genetics
  • Archaeal genetic
  • Metabolite engineering of bacteria


 

Working Experiences:

 

  • 2008: Biotechnology Research Center, Research Institute of Petroleum Industry (RIPI), Tehran, Iran.
  • 2006-2009: Teaching Microbiology in Department of Microbiology, School of Biology, College of Science, University of Tehran., Tehran, Iran
  • 2010: Presenting PCR (Standard and High GC content) and electrophoresis in "Bioinformatics, DNA Extraction and

Molecular Identification of Microorganisms" workshop. University of Tehran. Iran.

  • 2011 Working as a gust researcher in University of Tubingen, Germany
  • 2011-Teaching Microbiology in Department of Microbiology, School of Biology, College of Science, University of Tehran., Tehran, Iran
  • 2011-till now Teaching Microbial course  in Department of Microbiology, School of Biology, College of Science, University of Kharazmi., Tehran/Karaj, Iran

 

 

.

Teaching experience:

 

  • 2006-2008- Plant Microbiology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2006-2008- Health and Environmental Microbiology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2008-2009- Microbial Biology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2008-2009 and 2015-2016 Basic Microbiology 1 and 2, Department of Microbiology, School of Biology, College of Science, University of Tehran and Kharazmi, , Iran
  • 2009-2010- Microbial laboratory 1 and 2 Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2011-2016 Microbial laboratory 1 and 2 and General microbial laboratory Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2012-15 Food microbiology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2012 genetics of prokaryotes Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran  (master of science)
  • 2012 and 2016 Methods in Molecular Biology, Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2013-2016 Computer in Biology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran. (master of science)
  • 2014 and 2016 Virology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran.
  • 2016; Biotechnology, (Master of science)

 

Supervision:

  • 2013-2015… MS Student, School of Biotechnology, College of Science, University of Tehran., Tehran, Iran
  • 2013-2016 MS Student, Faculty of Biology, Molecular and cell Biology, Kharazmi university, Karaj, Iran
  • 2013-…PhD student, research center of genetics and biological products, Tehran Iran.
  • 2015-2017 MS Students, Faculty of Biology, College of Science, University of Tehran., Tehran, Iran
  •  
  •  
  • Reviewer:
  • In "Progress in Biology" journal
  • In "Nova Biologica Reperta" Journal

 

 

Skills:

 

Devices:

 

  • HPLC,
  • Spectrophotometer
  • Standard PCR
  • Electrophoresis
  • SDS-PAGE Electrophoresis
  • Protein purification column
  • Pulse field Electrophoresis devise
  • Real time PCR

 

Lab working:

  • PCR (Standard, High GC content, Touch Down, Soeing)
  • Restriction enzyme digestion
  • Ligase reaction
  • Transformation of E. coli
  • Protoplast preparation of Streptomyces. Lividans
  • DNA and Plasmid extraction
  • Primer Design (simple and degenerated one)
  • Gene Cloning in E. coli's vectors
  • Western blotting
  • SDS-PAGE
  • Phylogenic Analysis
  • Bacterial Screening from Soil and water
  • Assay of Enzyme Activity such as Lipase and protease
  • Optimization of Bacterial products with One Factor at a Time and Taguchi methods.
  • Protein purification
  • Gene expression (Rosetta Technology)
  • Southern Blotting
  • Pulse-field Electrophoresis
  • RNA extraction
  • cDNA synthesis
  • Real time PCR
  • Gene insertion deletion
  • Codon optimization and 2th mRNA structure

 

 

Computational software:

  • Clustal X,
  •  Mega,
  • Bioedit,
  • Oligo,
  • Chromas pro
  • Gene Runner
  • Taguchi
  • PatScan
  • EndNote
  • Gene script
  • Mfold
  • Prism

 

 

Publication:

 

Articles:

 

  • Mohammad Ali Amoozegar, Ensieh Salehghamari, Khosro Khajeh. Mahbubeh Kabiri. Production of an extracellular thermoalkalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2. Journal of Basic Microbiology, 2008, 48, 160-167. (in English), 1385
  • Mohammad Ali Amoozegar, Ensieh Salehghamari, Khosro Khajeh. Extracellular Lipase production and optimization in a native moderately halophilic bacterium of  Iran.2008. Iran Journal of Biology. Winter version.1386
  • Faezeh fallah ghahroodi, Javad Hamedi, Zargham Sepehrizadeh, Fatemeh Mohammadi panah, Hamid Moghimi, Ensieh Salehghamari, Molecular screening of non ribosomal peptid synthetase and study of antibiotic activity of two native isolated actinomycetes of Iran.  2011, Iranian knowledge of Biology, 6 (3) 55-65, 1390
  • Ensieh Salehghamari, Javad Hamedi, Elahe Elahi, Zargham Sepehrizadeh, Mehdi Sadeghi, Geunther Muth, Expression of the two-component response regulator phoP and characterization of PhoP target genes in Streptomyces clavuligerus DSM 738, New microbiologica, 2012, New Microbiologica,  (in English) 1391
  • Ensieh Salehghamari, Tafakori vida, , Antibacterial activity of some actinomycetes isolated from soils of Alborz province, Iran, 2015, Progress in Biological science Vol. 5, No. 2, 159-167.
  • Ensieh Salehghamari, Najafi Maryam. Isolation of biologically active Actinomycetes from untouched soils: a case study from Karaj district, Iran. Progress in Biological Sciences. 2016 Oct 1;6(1):65-74.
  • Ensieh Salehghamari, Amoozegar MA, Optimization of lipase production in Salinivibrio sp. SA2 by Taguchi design, Nova Biologica reperta, Accepted 2017.
  •  

 

Conferences:

  •  Hassan Tirandaz, Ensieh Salehghamari, Mohammad Ali Amoozegar. Screenng of native nucleases and lipases producing halophilic bacteria of Iran. International Conference on Extremophiles, 17-21 Sept. 2006. Brest, France.
  • Mohammad Ali Amoozegar, Ensieh Salehghamari and Khosro Khajeh. Optimization of lipase production in a new isolated moderately halophilic bacterium, Salinivibrio. International Conference on Extremophiles, 17-21 Sept. 2006. Brest, France.
  • E.Salehghamari, M.A.Amoozegar, K.Khajeh. Optimization of lipase production in a new native moderately halophilic bacterium of Iran. The 8th National congress of microbiology. 23-25 May 2006- Isfahan-Iran.
  • E.Salehghamari, M.A.Amoozegar, K.Khajeh. Optimization Of Lipase Production In A New Native Moderately Halophilic Bacterium Salinivibrio sp. Strain ZG13-2 By Taguchi Method.  14th  National and 2nd  International conference of Biology, Tehran, 29-31 August. 2006.
  • E. Salehghamari, Hamedi J, Elahi E. Sepehrizadeh Z, Partial sequence of phoR-phoP operon and phop gene cloning of Streptomyces clavuligerus DSM 738. Second National Conference of Applied Microbiology. 2011
  • E. Salehghamari, M. Sorayya, Antibacterial activity of some actinomycetes isolated from soils of Karaj, 18th national and 6th international Congress in biology in Iran, 2014
  • E. Salehghamari, Hamedi J, Elahi E., The copy number of phoP gene in Streptomyces clavuligerus chromosome. 18th national and 6th international Congress in biology in Iran, 2014
  • E. Salehghamari, Hamedi J, Elahi E., phoP deletion increase two secondary metabolite production in actinomycete model. 18th national and 6th international Congress in biology in Iran, 2014
  • Ensieh salehghamari, antibacterial activity of some isolated bacteria from irankaraj. The 16th international and Iranian congress of Microbiology, 2015

 

 

  • Morvarid Ebrahimi, Ensieh Salehghamari, Amir Salehi-Najafabadi, Mohammad Ali Amoozegar, Ghasem Amoabediny. Making ldha deletion construct to amplify isobutanol biosynthesis in escherichia coli The 16th international and Iranian congress of Microbiology. 2015

 

  • Zohre Nasrollahzade, Mohammad Tahmaseb, Ensieh Salehghamari, Mohammad Ali Amoozegar, bioassay and molecular screening pectinase enzyme in Halotolerant actinomycete, The 16th international and Iranian congress of Microbiology, 2015

 

  • Ensieh salehghamari, Najafi Maryam, جداسازی اکتینو میست­های فعال زیستی علیه باکتری استافیلوکوکوس اورئوس, 2th National congress of new cellular and molecular Biotechnology, 2015

 

 

  • Fatemeh Taheri, Marzieh Hosseini, Ensieh Salehghamari, Anti MRSA metabolites produced by co cultured actinomycetes isolated from Hoz-Sultan salt lake, Qom. The 17th international and Iranian congress of microbiology, 2016

 

  • Zohre Nasrollahzade, , Mohammad Tahmaseb, Ensieh Salehghamari, Mohammad Ali Amoozegar, A study of pectinase enzyme gene in halophilic bacteria from salt lake iran.  The 17th international and Iranian congress of microbiology, 2016

 

 

  • Full article presented at confreces:
  • Dariush Mahmoodi, Mohsen Rezaii, Javad Ashjari, Ensieh Salehghamari, Assesing transport of E. coli in porus media using 3-D physical model. 32th national and 1st international Geosciences congress.  ,2014
  • Marzieh Hosseini, Ensieh Salehghamari, Fatemeh Taheri, Evaluation of interspecies relationship effects of halophilic and halotolerant filamentous bacteria isolated from Hoz-Soltan lake on antibiotic production. The 17th international and Iranian congress of microbiology, 2016
  • Dariush Mahmoodi, Mohsen Rezaii, Javad Ashjari, Ensieh Salehghamari, Mirmasood kheirkhah Zarkesh, تحلیل فضایی انتقال آلاینده باکتریایی در محیط متخلخل اشباع با تأکید بر تغییرات جنس محیط,The 8th national conference and exhibition on environmental biology ,2016

 

 

Membership and Associations:

 

  • Society of Microbiology of Iran

    In the name of Allah (updated on 11-2-2017)

     

    Ensieh Salehghamari

     

    Assistant Professor in Microbiology

     

    Microbiology Lab.

    Dept. of Cell and Molecular Biology

    Faculty of Biological Science

    University of Kharazmi

    Karaj-Iran

    E-mail: ensiehsalehghamari@gmail.com

     

     

    Educational Background:

     

    (2006- 2012)                  PhD in Molecular Microbioloy at Department of Microbiology, School of Biology, College of Science, University of Tehran.

    (2003- 2006)                        Master of Science in Environmental Microbiology at Department of Microbiology, School of Biology, College of Science, University of Tehran.

    (1999-2003)                         Bachelors of Science in Microbiology at School of Biology, College of Science, University of Tehran.

     

     

    Academic Honors:

     

  • The first rank among graduates B.Sc. Microbiology, School of Biology, University of Tehran.
  • The third rank among graduates M.Sc. Microbiology, School of Biology, University of Tehran.
  • Second score in PhD exam of Tehran university
  • The Second rank among graduates PhD, Microbiology, School of Biology, University of Tehran


 

Scientific Interests:

 

  • Natural Products
  • Molecular microbiology
  • Actinomycete physiology and genetics
  • Archaeal genetic
  • Metabolite engineering of bacteria


 

Working Experiences:

 

  • 2008: Biotechnology Research Center, Research Institute of Petroleum Industry (RIPI), Tehran, Iran.
  • 2006-2009: Teaching Microbiology in Department of Microbiology, School of Biology, College of Science, University of Tehran., Tehran, Iran
  • 2010: Presenting PCR (Standard and High GC content) and electrophoresis in "Bioinformatics, DNA Extraction and

Molecular Identification of Microorganisms" workshop. University of Tehran. Iran.

  • 2011 Working as a gust researcher in University of Tubingen, Germany
  • 2011-Teaching Microbiology in Department of Microbiology, School of Biology, College of Science, University of Tehran., Tehran, Iran
  • 2011-till now Teaching Microbial course  in Department of Microbiology, School of Biology, College of Science, University of Kharazmi., Tehran/Karaj, Iran

 

 

.

Teaching experience:

 

  • 2006-2008- Plant Microbiology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2006-2008- Health and Environmental Microbiology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2008-2009- Microbial Biology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2008-2009 and 2015-2016 Basic Microbiology 1 and 2, Department of Microbiology, School of Biology, College of Science, University of Tehran and Kharazmi, , Iran
  • 2009-2010- Microbial laboratory 1 and 2 Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2011-2016 Microbial laboratory 1 and 2 and General microbial laboratory Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2012-15 Food microbiology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2012 genetics of prokaryotes Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran  (master of science)
  • 2012 and 2016 Methods in Molecular Biology, Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2013-2016 Computer in Biology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran. (master of science)
  • 2014 and 2016 Virology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran.
  • 2016; Biotechnology, (Master of science)

 

Supervision:

  • 2013-2015… MS Student, School of Biotechnology, College of Science, University of Tehran., Tehran, Iran
  • 2013-2016 MS Student, Faculty of Biology, Molecular and cell Biology, Kharazmi university, Karaj, Iran
  • 2013-…PhD student, research center of genetics and biological products, Tehran Iran.
  • 2015-2017 MS Students, Faculty of Biology, College of Science, University of Tehran., Tehran, Iran
  •  
  •  
  • Reviewer:
  • In "Progress in Biology" journal
  • In "Nova Biologica Reperta" Journal

 

 

Skills:

 

Devices:

 

  • HPLC,
  • Spectrophotometer
  • Standard PCR
  • Electrophoresis
  • SDS-PAGE Electrophoresis
  • Protein purification column
  • Pulse field Electrophoresis devise
  • Real time PCR

 

Lab working:

  • PCR (Standard, High GC content, Touch Down, Soeing)
  • Restriction enzyme digestion
  • Ligase reaction
  • Transformation of E. coli
  • Protoplast preparation of Streptomyces. Lividans
  • DNA and Plasmid extraction
  • Primer Design (simple and degenerated one)
  • Gene Cloning in E. coli's vectors
  • Western blotting
  • SDS-PAGE
  • Phylogenic Analysis
  • Bacterial Screening from Soil and water
  • Assay of Enzyme Activity such as Lipase and protease
  • Optimization of Bacterial products with One Factor at a Time and Taguchi methods.
  • Protein purification
  • Gene expression (Rosetta Technology)
  • Southern Blotting
  • Pulse-field Electrophoresis
  • RNA extraction
  • cDNA synthesis
  • Real time PCR
  • Gene insertion deletion
  • Codon optimization and 2th mRNA structure

 

 

Computational software:

  • Clustal X,
  •  Mega,
  • Bioedit,
  • Oligo,
  • Chromas pro
  • Gene Runner
  • Taguchi
  • PatScan
  • EndNote
  • Gene script
  • Mfold
  • Prism

 

 

Publication:

 

Articles:

 

  • Mohammad Ali Amoozegar, Ensieh Salehghamari, Khosro Khajeh. Mahbubeh Kabiri. Production of an extracellular thermoalkalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2. Journal of Basic Microbiology, 2008, 48, 160-167. (in English), 1385
  • Mohammad Ali Amoozegar, Ensieh Salehghamari, Khosro Khajeh. Extracellular Lipase production and optimization in a native moderately halophilic bacterium of  Iran.2008. Iran Journal of Biology. Winter version.1386
  • Faezeh fallah ghahroodi, Javad Hamedi, Zargham Sepehrizadeh, Fatemeh Mohammadi panah, Hamid Moghimi, Ensieh Salehghamari, Molecular screening of non ribosomal peptid synthetase and study of antibiotic activity of two native isolated actinomycetes of Iran.  2011, Iranian knowledge of Biology, 6 (3) 55-65, 1390
  • Ensieh Salehghamari, Javad Hamedi, Elahe Elahi, Zargham Sepehrizadeh, Mehdi Sadeghi, Geunther Muth, Expression of the two-component response regulator phoP and characterization of PhoP target genes in Streptomyces clavuligerus DSM 738, New microbiologica, 2012, New Microbiologica,  (in English) 1391
  • Ensieh Salehghamari, Tafakori vida, , Antibacterial activity of some actinomycetes isolated from soils of Alborz province, Iran, 2015, Progress in Biological science Vol. 5, No. 2, 159-167.
  • Ensieh Salehghamari, Najafi Maryam. Isolation of biologically active Actinomycetes from untouched soils: a case study from Karaj district, Iran. Progress in Biological Sciences. 2016 Oct 1;6(1):65-74.
  • Ensieh Salehghamari, Amoozegar MA, Optimization of lipase production in Salinivibrio sp. SA2 by Taguchi design, Nova Biologica reperta, Accepted 2017.
  •  

 

Conferences:

  •  Hassan Tirandaz, Ensieh Salehghamari, Mohammad Ali Amoozegar. Screenng of native nucleases and lipases producing halophilic bacteria of Iran. International Conference on Extremophiles, 17-21 Sept. 2006. Brest, France.
  • Mohammad Ali Amoozegar, Ensieh Salehghamari and Khosro Khajeh. Optimization of lipase production in a new isolated moderately halophilic bacterium, Salinivibrio. International Conference on Extremophiles, 17-21 Sept. 2006. Brest, France.
  • E.Salehghamari, M.A.Amoozegar, K.Khajeh. Optimization of lipase production in a new native moderately halophilic bacterium of Iran. The 8th National congress of microbiology. 23-25 May 2006- Isfahan-Iran.
  • E.Salehghamari, M.A.Amoozegar, K.Khajeh. Optimization Of Lipase Production In A New Native Moderately Halophilic Bacterium Salinivibrio sp. Strain ZG13-2 By Taguchi Method.  14th  National and 2nd  International conference of Biology, Tehran, 29-31 August. 2006.
  • E. Salehghamari, Hamedi J, Elahi E. Sepehrizadeh Z, Partial sequence of phoR-phoP operon and phop gene cloning of Streptomyces clavuligerus DSM 738. Second National Conference of Applied Microbiology. 2011
  • E. Salehghamari, M. Sorayya, Antibacterial activity of some actinomycetes isolated from soils of Karaj, 18th national and 6th international Congress in biology in Iran, 2014
  • E. Salehghamari, Hamedi J, Elahi E., The copy number of phoP gene in Streptomyces clavuligerus chromosome. 18th national and 6th international Congress in biology in Iran, 2014
  • E. Salehghamari, Hamedi J, Elahi E., phoP deletion increase two secondary metabolite production in actinomycete model. 18th national and 6th international Congress in biology in Iran, 2014
  • Ensieh salehghamari, antibacterial activity of some isolated bacteria from irankaraj. The 16th international and Iranian congress of Microbiology, 2015

 

 

  • Morvarid Ebrahimi, Ensieh Salehghamari, Amir Salehi-Najafabadi, Mohammad Ali Amoozegar, Ghasem Amoabediny. Making ldha deletion construct to amplify isobutanol biosynthesis in escherichia coli The 16th international and Iranian congress of Microbiology. 2015

 

  • Zohre Nasrollahzade, Mohammad Tahmaseb, Ensieh Salehghamari, Mohammad Ali Amoozegar, bioassay and molecular screening pectinase enzyme in Halotolerant actinomycete, The 16th international and Iranian congress of Microbiology, 2015

 

  • Ensieh salehghamari, Najafi Maryam, جداسازی اکتینو میست­های فعال زیستی علیه باکتری استافیلوکوکوس اورئوس, 2th National congress of new cellular and molecular Biotechnology, 2015

 

 

  • Fatemeh Taheri, Marzieh Hosseini, Ensieh Salehghamari, Anti MRSA metabolites produced by co cultured actinomycetes isolated from Hoz-Sultan salt lake, Qom. The 17th international and Iranian congress of microbiology, 2016

 

  • Zohre Nasrollahzade, , Mohammad Tahmaseb, Ensieh Salehghamari, Mohammad Ali Amoozegar, A study of pectinase enzyme gene in halophilic bacteria from salt lake iran.  The 17th international and Iranian congress of microbiology, 2016

 

 

  • Full article presented at confreces:
  • Dariush Mahmoodi, Mohsen Rezaii, Javad Ashjari, Ensieh Salehghamari, Assesing transport of E. coli in porus media using 3-D physical model. 32th national and 1st international Geosciences congress.  ,2014
  • Marzieh Hosseini, Ensieh Salehghamari, Fatemeh Taheri, Evaluation of interspecies relationship effects of halophilic and halotolerant filamentous bacteria isolated from Hoz-Soltan lake on antibiotic production. The 17th international and Iranian congress of microbiology, 2016
  • Dariush Mahmoodi, Mohsen Rezaii, Javad Ashjari, Ensieh Salehghamari, Mirmasood kheirkhah Zarkesh, تحلیل فضایی انتقال آلاینده باکتریایی در محیط متخلخل اشباع با تأکید بر تغییرات جنس محیط,The 8th national conference and exhibition on environmental biology ,2016

 

 

Membership and Associations:

 

  • Society of Microbiology of Iran

    In the name of Allah (updated on 11-2-2017)

     

    Ensieh Salehghamari

     

    Assistant Professor in Microbiology

     

    Microbiology Lab.

    Dept. of Cell and Molecular Biology

    Faculty of Biological Science

    University of Kharazmi

    Karaj-Iran

    E-mail: ensiehsalehghamari@gmail.com

     

     

    Educational Background:

     

    (2006- 2012)                  PhD in Molecular Microbioloy at Department of Microbiology, School of Biology, College of Science, University of Tehran.

    (2003- 2006)                        Master of Science in Environmental Microbiology at Department of Microbiology, School of Biology, College of Science, University of Tehran.

    (1999-2003)                         Bachelors of Science in Microbiology at School of Biology, College of Science, University of Tehran.

     

     

    Academic Honors:

     

  • The first rank among graduates B.Sc. Microbiology, School of Biology, University of Tehran.
  • The third rank among graduates M.Sc. Microbiology, School of Biology, University of Tehran.
  • Second score in PhD exam of Tehran university
  • The Second rank among graduates PhD, Microbiology, School of Biology, University of Tehran


 

Scientific Interests:

 

  • Natural Products
  • Molecular microbiology
  • Actinomycete physiology and genetics
  • Archaeal genetic
  • Metabolite engineering of bacteria


 

Working Experiences:

 

  • 2008: Biotechnology Research Center, Research Institute of Petroleum Industry (RIPI), Tehran, Iran.
  • 2006-2009: Teaching Microbiology in Department of Microbiology, School of Biology, College of Science, University of Tehran., Tehran, Iran
  • 2010: Presenting PCR (Standard and High GC content) and electrophoresis in "Bioinformatics, DNA Extraction and

Molecular Identification of Microorganisms" workshop. University of Tehran. Iran.

  • 2011 Working as a gust researcher in University of Tubingen, Germany
  • 2011-Teaching Microbiology in Department of Microbiology, School of Biology, College of Science, University of Tehran., Tehran, Iran
  • 2011-till now Teaching Microbial course  in Department of Microbiology, School of Biology, College of Science, University of Kharazmi., Tehran/Karaj, Iran

 

 

.

Teaching experience:

 

  • 2006-2008- Plant Microbiology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2006-2008- Health and Environmental Microbiology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2008-2009- Microbial Biology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2008-2009 and 2015-2016 Basic Microbiology 1 and 2, Department of Microbiology, School of Biology, College of Science, University of Tehran and Kharazmi, , Iran
  • 2009-2010- Microbial laboratory 1 and 2 Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  • 2011-2016 Microbial laboratory 1 and 2 and General microbial laboratory Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2012-15 Food microbiology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2012 genetics of prokaryotes Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran  (master of science)
  • 2012 and 2016 Methods in Molecular Biology, Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran
  • 2013-2016 Computer in Biology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran. (master of science)
  • 2014 and 2016 Virology Department of Microbiology, School of Biology, College of Science, University of of Kharazmi., Tehran/Karaj, Iran.
  • 2016; Biotechnology, (Master of science)

 

Supervision:

  • 2013-2015… MS Student, School of Biotechnology, College of Science, University of Tehran., Tehran, Iran
  • 2013-2016 MS Student, Faculty of Biology, Molecular and cell Biology, Kharazmi university, Karaj, Iran
  • 2013-…PhD student, research center of genetics and biological products, Tehran Iran.
  • 2015-2017 MS Students, Faculty of Biology, College of Science, University of Tehran., Tehran, Iran
  •  
  •  
  • Reviewer:
  • In "Progress in Biology" journal
  • In "Nova Biologica Reperta" Journal

 

 

Skills:

 

Devices:

 

  • HPLC,
  • Spectrophotometer
  • Standard PCR
  • Electrophoresis
  • SDS-PAGE Electrophoresis
  • Protein purification column
  • Pulse field Electrophoresis devise
  • Real time PCR

 

Lab working:

  • PCR (Standard, High GC content, Touch Down, Soeing)
  • Restriction enzyme digestion
  • Ligase reaction
  • Transformation of E. coli
  • Protoplast preparation of Streptomyces. Lividans
  • DNA and Plasmid extraction
  • Primer Design (simple and degenerated one)
  • Gene Cloning in E. coli's vectors
  • Western blotting
  • SDS-PAGE
  • Phylogenic Analysis
  • Bacterial Screening from Soil and water
  • Assay of Enzyme Activity such as Lipase and protease
  • Optimization of Bacterial products with One Factor at a Time and Taguchi methods.
  • Protein purification
  • Gene expression (Rosetta Technology)
  • Southern Blotting
  • Pulse-field Electrophoresis
  • RNA extraction
  • cDNA synthesis
  • Real time PCR
  • Gene insertion deletion
  • Codon optimization and 2th mRNA structure

 

 

Computational software:

  • Clustal X,
  •  Mega,
  • Bioedit,
  • Oligo,
  • Chromas pro
  • Gene Runner
  • Taguchi
  • PatScan
  • EndNote
  • Gene script
  • Mfold
  • Prism

 

 

Publication:

 

Articles:

 

  • Mohammad Ali Amoozegar, Ensieh Salehghamari, Khosro Khajeh. Mahbubeh Kabiri. Production of an extracellular thermoalkalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2. Journal of Basic Microbiology, 2008, 48, 160-167. (in English), 1385
  • Mohammad Ali Amoozegar, Ensieh Salehghamari, Khosro Khajeh. Extracellular Lipase production and optimization in a native moderately halophilic bacterium of  Iran.2008. Iran Journal of Biology. Winter version.1386
  • Faezeh fallah ghahroodi, Javad Hamedi, Zargham Sepehrizadeh, Fatemeh Mohammadi panah, Hamid Moghimi, Ensieh Salehghamari, Molecular screening of non ribosomal peptid synthetase and study of antibiotic activity of two native isolated actinomycetes of Iran.  2011, Iranian knowledge of Biology, 6 (3) 55-65, 1390
  • Ensieh Salehghamari, Javad Hamedi, Elahe Elahi, Zargham Sepehrizadeh, Mehdi Sadeghi, Geunther Muth, Expression of the two-component response regulator phoP and characterization of PhoP target genes in Streptomyces clavuligerus DSM 738, New microbiologica, 2012, New Microbiologica,  (in English) 1391
  • Ensieh Salehghamari, Tafakori vida, , Antibacterial activity of some actinomycetes isolated from soils of Alborz province, Iran, 2015, Progress in Biological science Vol. 5, No. 2, 159-167.
  • Ensieh Salehghamari, Najafi Maryam. Isolation of biologically active Actinomycetes from untouched soils: a case study from Karaj district, Iran. Progress in Biological Sciences. 2016 Oct 1;6(1):65-74.
  • Ensieh Salehghamari, Amoozegar MA, Optimization of lipase production in Salinivibrio sp. SA2 by Taguchi design, Nova Biologica reperta, Accepted 2017.
  •  

 

Conferences:

  •  Hassan Tirandaz, Ensieh Salehghamari, Mohammad Ali Amoozegar. Screenng of native nucleases and lipases producing halophilic bacteria of Iran. International Conference on Extremophiles, 17-21 Sept. 2006. Brest, France.
  • Mohammad Ali Amoozegar, Ensieh Salehghamari and Khosro Khajeh. Optimization of lipase production in a new isolated moderately halophilic bacterium, Salinivibrio. International Conference on Extremophiles, 17-21 Sept. 2006. Brest, France.
  • E.Salehghamari, M.A.Amoozegar, K.Khajeh. Optimization of lipase production in a new native moderately halophilic bacterium of Iran. The 8th National congress of microbiology. 23-25 May 2006- Isfahan-Iran.
  • E.Salehghamari, M.A.Amoozegar, K.Khajeh. Optimization Of Lipase Production In A New Native Moderately Halophilic Bacterium Salinivibrio sp. Strain ZG13-2 By Taguchi Method.  14th  National and 2nd  International conference of Biology, Tehran, 29-31 August. 2006.
  • E. Salehghamari, Hamedi J, Elahi E. Sepehrizadeh Z, Partial sequence of phoR-phoP operon and phop gene cloning of Streptomyces clavuligerus DSM 738. Second National Conference of Applied Microbiology. 2011
  • E. Salehghamari, M. Sorayya, Antibacterial activity of some actinomycetes isolated from soils of Karaj, 18th national and 6th international Congress in biology in Iran, 2014
  • E. Salehghamari, Hamedi J, Elahi E., The copy number of phoP gene in Streptomyces clavuligerus chromosome. 18th national and 6th international Congress in biology in Iran, 2014
  • E. Salehghamari, Hamedi J, Elahi E., phoP deletion increase two secondary metabolite production in actinomycete model. 18th national and 6th international Congress in biology in Iran, 2014
  • Ensieh salehghamari, antibacterial activity of some isolated bacteria from irankaraj. The 16th international and Iranian congress of Microbiology, 2015

 

 

  • Morvarid Ebrahimi, Ensieh Salehghamari, Amir Salehi-Najafabadi, Mohammad Ali Amoozegar, Ghasem Amoabediny. Making ldha deletion construct to amplify isobutanol biosynthesis in escherichia coli The 16th international and Iranian congress of Microbiology. 2015

 

  • Zohre Nasrollahzade, Mohammad Tahmaseb, Ensieh Salehghamari, Mohammad Ali Amoozegar, bioassay and molecular screening pectinase enzyme in Halotolerant actinomycete, The 16th international and Iranian congress of Microbiology, 2015

 

  • Ensieh salehghamari, Najafi Maryam, جداسازی اکتینو میست­های فعال زیستی علیه باکتری استافیلوکوکوس اورئوس, 2th National congress of new cellular and molecular Biotechnology, 2015

 

 

  • Fatemeh Taheri, Marzieh Hosseini, Ensieh Salehghamari, Anti MRSA metabolites produced by co cultured actinomycetes isolated from Hoz-Sultan salt lake, Qom. The 17th international and Iranian congress of microbiology, 2016

 

  • Zohre Nasrollahzade, , Mohammad Tahmaseb, Ensieh Salehghamari, Mohammad Ali Amoozegar, A study of pectinase enzyme gene in halophilic bacteria from salt lake iran.  The 17th international and Iranian congress of microbiology, 2016

 

 

  • Full article presented at confreces:
  • Dariush Mahmoodi, Mohsen Rezaii, Javad Ashjari, Ensieh Salehghamari, Assesing transport of E. coli in porus media using 3-D physical model. 32th national and 1st international Geosciences congress.  ,2014
  • Marzieh Hosseini, Ensieh Salehghamari, Fatemeh Taheri, Evaluation of interspecies relationship effects of halophilic and halotolerant filamentous bacteria isolated from Hoz-Soltan lake on antibiotic production. The 17th international and Iranian congress of microbiology, 2016
  • Dariush Mahmoodi, Mohsen Rezaii, Javad Ashjari, Ensieh Salehghamari, Mirmasood kheirkhah Zarkesh, تحلیل فضایی انتقال آلاینده باکتریایی در محیط متخلخل اشباع با تأکید بر تغییرات جنس محیط,The 8th national conference and exhibition on environmental biology ,2016

 

 

Membership and Associations:

 

  • Society of Microbiology of Iran